At last night's American Music Awards, Jennifer Lopez opened the show by dancing to a medley of hits by other artists.
One of the songs in the epic mashup was Nicki Minaj's "Anaconda."
If anyone can do justice to Nicki's ode to the booty, it's J-Lo - but apparently not everyone feels that way.
Cameras picked the perfect time to zoom in on Nicki's face during the performance, and as you can see in the clip below, Ms. Minaj was not amused.
Perhaps Nicki believes that the song was meant for her ass and hers alone.
Or maybe she's just trying to secure her spot as the Queen of Awards Show Beefs.
As you may recall, Nicki kicked off a feud with Miley Cyrus at the VMAs back in August. Now it seems J-Lo is the latest target of her wrath.
Of course, this time Nicki's outrage is much more subdued, but we don't think there's any mistaking the look on that face.
The rest of the night saw Nicki smiling and seeming to enjoy herself with boyfriend Meek Mill by her side, so it seems her sour mood didn't last.
Even so, Jenny From the Block should probably think twice before she attempts to pay tribute to Nicki again.