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Even at 53, Madonna is clearly not afraid to go there.

During her concert in Istanbul this weekend, the Queen of Pop tantalized the crowd during a performance of her hit song “Human Nature,” wearing a skimpy bra top that she promptly pulled down to reveal one voluptuous nipple.

This was no wardrobe malfunction, either.

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This is Madge basically saying “yes, I can and will do this … deal.”

As the decades go by, Madonna has shown no signs of slowing down, or going soft. She continues confront critics – with an absence of subtlety – who say she goes too far in invoking sex in her music and performances.

She’s still finding an audience for it, too.

Her big MDNA concert stop in Istanbul came before 55,000 strong, and she reportedly followed up “Human Nature” with “Like A Virgin” … natch.